Home / Equipment / Titan Krios (FEI)

Contact

Dewight Williams
Associate Research Scientist
dwilli76@asu.edu




Titan Krios (FEI)

The Titan Krios is a dedicated cryogenic electron microscope for life science imaging of cellular and macromolecular assemblies.

It possesses a highly stable cryogenic environment suitable for automated and semi-automated data acquisition. Applications include 2D electron crystallography, single particle imaging, and tomographic imaging. It has a proven performance at obtaining images that preserve near atomic resolution detail in biological material in combination with second-generation direct electron detectors. The Krios is also equipped with FEI Volta phase plate technology that provides increased contrast to biological material embedded in vitreous ice.

Specifications:

  • Autoloader with 12 sample positions
  • X-FEG emission source
  • Phase plates
  • K2 Summit direct electron detector
  • Ceta* hybrid CMOS camera for electron diffraction data collection
  • STEM and HADDF detector
  • Constant current lenses and easy parallel beam illumination
  • Serial EM, EPU, S/TEM tomography software for automated data collection

Techniques: Cryogenic TEM 

Biological material preserved in vitreous ice by rapid freezing methods provides the nearest to native preservation of biological molecules. In conjunction with tomographic or single particle reconstruction methods, molecular and atomic resolution models of the macromolecular complexes can be obtained. The facility also houses preparatory equipment for plunge freezing, sample screening, and grid preparation.  Available in house are carbon evaporators, glow dischargers, grid preparation materials, and a manual and automated (Vitrobot) plunge freezers. Liquid nitrogen storage for short-term holding is also available.

Consultation is required prior to starting a project within the facility. Samples submitted for cryoTEM imaging on the Titan Krios need to be accompanied with appropriate documentation establishing the sample is suitable for imaging. This is either a set of cryoTEM images establishing monodispersed particles in appropriate ice thickness and/or an initial structure derived from either negative stain TEM or lower resolution cryoTEM studies. Samples prepared in house will be screened by negative stain to demonstrate suitability for single particle imaging prior to cryogenic preparation by plunge freezing.

Shipping in a dry shipper is best, but appropriate packaging of the grid boxes must be performed to avoid frost deposition during transport. 

Rates

Cost for ASU Internal Cost for ASU Internal with Staff Assistance Cost for Other Academic/Non-Profit Cost for Other Academic/Non-Profit with Staff Assistance
$600/day $38/h+day $1000/day $80/h + day

Training Requirements

Safety

Emergency Information

See ASU Emergency Procedures and Information for emergency procedures for any of the following: Personal Threat or Assault, Power Outage, Major Accidents, Flooding, Hazardous Materials Incident, Radiation Spills, Biological Spills, Suspicious Packages, Natural Disaster & Inclement Weather, Bomb Threat, Evacuation, Medical Emergency, Personal Injury, and Fire.

Emergency Phone Numbers

In an emergency, dial 911 from any campus or other phone.

Call the General ASU Emergency Information Phone at 7-9911 (on-campus phone) or 480-727-9911 or toll-free 877-278-2785 (877-ASU-ASU5) to get a recorded message. For non-emergencies, The ASU Department of Public Safety office can be reached at 5-3465 (on-campus phone) or 480-965-3465.

Users must attend the ASU Lab Safety and Fire Safety courses prior to using any LE-CSSS facility.

Examples

More Information

Active users can schedule time using their iLab account with the following link Titan Krios Schedule

If you are not currently in iLab please click on the following link: iLab registration

  • Reserving time on an instrument means that you agree to pay for the entire time reserved at the standard rate whether you use it or not. This policy ensures the efficient use of instrumentation and discourages researchers from blocking up time that they are not going to use. You will also be billed for any time used that exceeds your reservation.